Demonstration of LDH-X for identification of testicular tissues.

The sperm-specific lactate dehydrogenase isoenzyme (LDH-X) appearing in electrophoresis between LDH-3 and LDH-4 is characteristic of human mature testis and spermatozoa. 1,21 Our previous studies have shown that detection of LDH-X by starch gel electrophoresis is quite suitable for forensic demonstration of semen from vaginal swabs. ) This communication reports the successful application of the LDH isoenzyme method for determining the origin of a specimen adherent to the muffler of a car in a traffic accident and the results of some supplementary experiments.